磷酸化干扰素调节因子7抗体

英文名称Phospho-IRF7 (Ser471 + Ser472)
中文名称磷酸化干扰素调节因子7抗体
别 名Interferon regulatory factor 7; Interferon regulatory factor 7H; IRF 7; IRF 7A; IRF 7H; IRF7A; IRF7; IRF7H.
规格100ul
文献有引用：

产品类型磷酸化抗体
研究领域肿瘤 信号转导 细胞凋亡 转录调节因子
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Pig, Cow, Horse,
产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 Flow-Cyt=1ug/test （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量54kDa
细胞定位细胞核 细胞浆
性 状Lyophilized or Liquid
浓 度1mg/ml
免 疫 原KLH conjugated synthesised phosphopeptide derived from human IRF7 around the phosphorylation site of Ser471/472:GV(p-S)(p-S)LD
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
IRF7 encodes interferon regulatory factor 7, a member of the interferon regulatory transcription factor (IRF) family. IRF7 has been shown to play a role in the transcriptional activation of virus-inducible cellular genes, including interferon beta chain genes. Inducible expression of IRF7 is largely restricted to lymphoid tissue. Multiple IRF7 transcript variants have been identified, although the functional consequences of these have not yet been established. [provided by RefSeq, Jul 2008]

Function:
Transcriptional activator. Binds to the interferon-stimulated response element (ISRE) in IFN promoters and in the Q promoter (Qp) of EBV nuclear antigen 1 (EBNA1). Functions as a molecular switch for antiviral activity. Activated by phosphorylation in response to infection. Activation leads to nuclear retention, DNA binding, and derepression of transactivation ability.

Subunit:
Homodimer.

Subcellular Location:
Nucleus. Cytoplasm. The phosphorylated and active form accumulates selectively in the nucleus.

Tissue Specificity:
Expressed predominantly in spleen, thymus and peripheral blood leukocytes.

Post-translational modifications:
In response to a viral infection, phosphorylated on the C-terminal serine cluster. Phosphorylation, and subsequent activation is inhibited by vaccinia virus protein E3.
TRAF6-mediated ubiquitination is required for IRF7 activation.

Similarity:
Belongs to the IRF family.
Contains 1 IRF tryptophan pentad repeat DNA-binding domain.

SWISS:
Q92985

Gene ID:
3665

Database links:
Entrez Gene: 3665 Human
Entrez Gene: 54123 Mouse
Entrez Gene: 293624 Rat
Omim: 605047 Human
SwissProt: Q92985 Human
SwissProt: P70434 Mouse
Unigene: 166120 Human
Unigene: 3233 Mouse
Unigene: 101159 Rat


Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.



Sample:
Uterus (Mouse) Lysate at 40 ug
Large intestine (Mouse) Lysate at 40 ug
Primary: Anti-Phospho-IRF7 (Ser471 + Ser472)(bs-3196R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54kD
Observed band size: 49kD


Sample:Cerebrum (Rat) Lysate at 40 ug
Primary: Anti-Phospho-IRF7 (Ser471 + Ser472)(bs-3196R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54kD
Observed band size: 48kD


Sample:Spleen (Mouse) Lysate at 40 ug
Primary: Anti-Phospho-IRF7 (Ser471 + Ser472)(bs-3196R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54kD
Observed band size: 49kD


Sample: Heart(Mouse) lysate at 30ug;
Primary: Anti-Phospho-IRF7 (Ser471+Ser472) (bs-3196R) at 1:200 dilution;
Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution;
Predicted band size : 54kD
Observed band size : 51kD


Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-IRF7 (Ser471 + Ser472)) Polyclonal Antibody, Unconjugated (bs-3196R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-IRF7 (Ser471 + Ser472)) Polyclonal Antibody, Unconjugated (bs-3196R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Blank control:Molt-4.
Primary Antibody (green line): Rabbit Anti-Phospho-IRF7 (Ser471 + Ser472) antibody (bs-3196R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.