磷酸化真核启动因子2α抗体

英文名称Phospho-eIF2 alpha(Ser51)
中文名称磷酸化真核启动因子2α抗体
别 名EIF2S1 (phospho S51); EIF2S1 (phospho-Ser51); CDA 02; CDA02; eIF 2 alpha; EIF 2; EIF 2A; EIF-2alpha; EIF2; EIF2A; EIF2alpha; eIF2S1; Eukaryotic Translation Initiation Factor 2 alpha; Eukaryotic translation initiation factor 2 subunit 1 alpha 35kDa; Eukaryotic translation initiation factor 2 subunit alpha.
Specific References (1) | bs-4842R has been referenced in 1 publications.[IF=2.33] He, Yihuai, et al. "Sustained endoplasmic reticulum stress inhibits hepatocyte proliferation via downregulation of c-Met expression." Molecular and Cellular Biochemistry (2014): 1-8. WB ; Human. PubMed:24390087
规格价格100ul
产品类型磷酸化抗体
研究领域肿瘤 细胞生物 神经生物学 信号转导 细胞凋亡 转录调节因子 激酶和磷酸酶
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Cow,
产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量36kDa
细胞定位细胞浆
性 状Lyophilized or Liquid
浓 度1mg/ml
免 疫 原KLH conjugated synthesised phosphopeptide derived from human eIF2 alpha around the phosphorylation site of Ser51 [EL(p-S)RR]:EL(p-S)RR
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM, Feb 2010].

Function:
Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.

Subunit:
Heterotrimer composed of an alpha, a beta and a gamma chain. Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5. Interaction with METAP2 protects EIF2S1 from inhibitory phosphorylation. Interacts with ABCF1 isoform 2. Associates with ribosomes.

Subcellular Location:
Cytoplasmic granule. Note=The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Co-localizes with NANOS3 in the stress granules.

Post-translational modifications:
Substrate for at least 4 kinases: EIF2AK3/PERK, GCN2, HRI and PKR. Phosphorylation stabilizes the eIF-2/GDP/eIF-2B complex and prevents GDP/GTP exchange reaction, thus impairing the recycling of eIF-2 between successive rounds of initiation and leading to global inhibition of translation. In case of infection by vaccinia virus or rotavirus A, eIF2S1 phosphorylation state is modulated.

Similarity:
Belongs to the eIF-2-alpha family.
Contains 1 S1 motif domain.

SWISS:
P05198

Gene ID:
1965

Database links:
Entrez Gene: 1965 Human
Entrez Gene: 13665 Mouse
Entrez Gene: 54318 Rat
Omim: 603907 Human
SwissProt: P05198 Human
SwissProt: Q6ZWX6 Mouse
SwissProt: P68101 Rat
Unigene: 151777 Human
Unigene: 196220 Mouse
Unigene: 1488 Rat


Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

Sample: Testis (Mouse) Lysate at 40 ug
Primary: Anti-Phospho-eIF2 alpha(Ser51) (bs-4842R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kD
Observed band size: 34 kD


Sample: Ovary (Mouse) Lysate at 40 ug
Primary: Anti-Phospho-eIF2 alpha(Ser51) (bs-4842R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kD
Observed band size: 34 kD


Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (Phospho-eIF2 alpha(Ser51)) Polyclonal Antibody, Unconjugated (bs-4842R) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (Phospho-eIF2 alpha(Ser51)) Polyclonal Antibody, Unconjugated (bs-4842R) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.

Blank control(blue): U-87MG(fixed with 2% paraformaldehyde (10 min), then permeabilized with 90% ice-cold methanol for 30 min on ice).
Primary Antibody:Rabbit Anti-Phospho-eIF2 alpha(Ser51) antibody(bs-4842R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.