环氧合酶2/前列腺素内过氧化物合成酶2抗体

英文名称Cyclooxygenase 2
中文名称环氧合酶2/前列腺素内过氧化物合成酶2抗体
别 名COX 2; COX2; COX-2; Cyclooxygenase; Cyclooxygenase2; hCox 2; PGG/HS; PGH synthase 2; PGHS 2; PGHS2; PHS 2; PHS II; PHS2 ; Prostaglandin endoperoxide synthase 2; Prostaglandin G/H synthase 2 precursor; Prostaglandin G/H synthase and cyclooxygenase; Prostaglandin G/H synthase; Prostaglandin H2 synthase 2; PTGS2; TIS10II; PGH2_HUMAN.
文献引用如下：
Specific References (10) | bs-0732R has been referenced in 10 publications.[IF=2.55] Zhang, Jing-Yao, et al. "Protective role of hydrogen-rich water on aspirin-induced gastric mucosal damage in rats." World J Gastroenterol 20.6 (2014): 1614-1622. IHC-P ; Rat. PubMed:n/a [IF=2.42] Li, Weifeng, et al. "Protective effect of sanguinarine on LPS-induced endotoxic shock in mice and its effect on LPS-induced COX-2 expression and COX-2 associated PGE< sub> 2 release from peritoneal macrophages." International Immunopharmacology (2014). IHC-P ; Mouse. PubMed:25063710 [IF=1.84] de Moraes, Carolina Nogueira, et al. "Temporal analysis of FP receptor, CASP-3 and COX-2 mRNA expression and FP receptor and COX-2 protein expression in endometrial tissue from multiparous Nelore (< i> Bos taurus indicus) cows treated with cloprostenol sodium during puerperium." Theriogenology (2014). IHC-P ; Bovine. PubMed:25442016 [IF=2.38] Hu, Ruiqi, et al. "The expression of prostaglandin-E2 and its receptor in the oviduct of Chinese brown frog (Rana dybowskii)." Prostaglandins & Other Lipid Mediators (2016). IHC-P ; Others. PubMed:27246901 [IF=2.86] Li, Wei, et al. "Combined effects of cyclooxygenase-1 and cyclooxygenase-2 selective inhibitors on ovarian carcinoma in vivo." International journal of molecular sciences 12.1 (2011): 668-681. WB ; Mouse. PubMed:21340007 [IF=4.82] Fattori, Victor, et al. "Vinpocetine reduces diclofenac-induced acute kidney injury through inhibition of oxidative stress, apoptosis, cytokine production, and NF-κB activation in mice." Pharmacological Research (2017). IHC-P ; Mouse. PubMed:0 [IF=3.25] Li, Wei, et al. "Cyclin d1 expression and the inhibitory effect of celecoxib on ovarian tumor growth in vivo." International journal of molecular sciences 11.10 (2010): 3999-4013. WB ; Human. PubMed:21152316 [IF=0.44] Sutrisno, Sutrisno, et al. "The effect of genistein on TGF-β signal, dysregulation of apoptosis, cyclooxygenase-2 pathway, and NF-kB pathway in mice peritoneum of endometriosis model." Middle East Fertility Society Journal (2017). IHC-P ; Mouse. PubMed:0 [IF=3.73] Gao, Jin-Hang, et al. "Celecoxib Ameliorates Portal Hypertension of the Cirrhotic Rats through the Dual Inhibitory Effects on the Intrahepatic Fibrosis and Angiogenesis." PLOS ONE 8.7 (2013): e69309. WB ; Rat. PubMed:23922700 [IF=2.02] Chu, Changbin, et al. "Celastrol protects kidney against ischemia–reperfusion-induced injury in rats." Journal of Surgical Research (2013). IHC-P ; Rat. PubMed:23981709
规格价格50ul/780元 购买 100ul/1380元 购买 200ul/2200元 购买 大包装/询价
说 明 书50ul 100ul 200ul
研究领域肿瘤 免疫学 生长因子和激素 合成与降解
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, Sheep,
产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量65kDa
细胞定位细胞浆
性 状Lyophilized or Liquid
浓 度1mg/ml
免 疫 原KLH conjugated synthetic peptide derived from human Cyclooxygenase 2:501-604/604
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes the inducible isozyme. It is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis. [provided by RefSeq, Feb 2009]

Function:
Mediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity.

Subunit:
Homodimer.

Subcellular Location:
Microsome membrane; Peripheral membrane protein. Endoplasmic reticulum membrane; Peripheral membrane protein.

Post-translational modifications:
S-nitrosylation by NOS2 (iNOS) activates enzme activity. S-nitrosylation may take place on different Cys residues in addition to Cys-561.

Similarity:
Belongs to the prostaglandin G/H synthase family. Contains 1 EGF-like domain.

SWISS:
Q05769

Gene ID:
5743

Database links:
Entrez Gene: 5743 Human
Entrez Gene: 19225 Mouse
Entrez Gene: 29527 Rat
Omim: 600262 Human
SwissProt: P35354 Human
SwissProt: Q05769 Mouse
SwissProt: P35355 Rat
Unigene: 196384 Human
Unigene: 292547 Mouse
Unigene: 44369 Rat


Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

合成与降解（Synthesis and Degradation）
COX-2 前列腺素氧化环化酶-2是前列腺素合成的关键酶之一，为诱导性酶，他参与细胞对生长因子、肿瘤促进因子和细胞因子的反应，这些因子均能诱导COX2表达，它也参与前列腺素的合成。
COX2在80-90％的人结肠腺癌中表达显著增高，而COX1表达却不变。

产品图片

Sample: Brain (Mouse) Lysate at 30 ug Primary: Anti- Cyclooxygenase 2 (bs-0732R) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution Predicted band size: 65 kD Observed band size: 62 kD Sample: A549 Cell Lysate at 30 ug Primary: Anti- Cyclooxygenase 2 (bs-0732R) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution Predicted band size: 65 kD Observed band size: 62 kD Sample: Lung (Mouse) Lysate at 40 ug Primary: Anti-Cyclooxygenase 2 (bs-0732R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 65 kD Observed band size: 65 kD Sample: A549(Human) Cell Lysate at 30 ug Primary: Anti-Cyclooxygenase 2 (bs-0732R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 65 kD Observed band size: 70 kD Tissue/cell: rat brain; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃for 20 min; Incubation: Anti-Cyclooxygenase 2 Polyclonal Antibody, Unconjugated(bs-0732R) 1:400, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Blank control (blue line): HepG2 (blue). Primary Antibody (green line): Rabbit Anti-Cyclooxygenase 2/FITC Conjugated antibody (bs-0732R-FITC) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG-FITC . Protocol The cells were fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature.Acquisition of 20,000 events was performed. Blank control (blue line): HepG2 (blue). Primary Antibody (green line): Rabbit Anti-Cyclooxygenase 2/FITC Conjugated antibody (bs-0732R-FITC) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG-FITC . Protocol The cells were fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature.Acquisition of 20,000 events was performed.