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文献和实验96-well RNA In Situ Hybridization Protocol
Noordermeer and Casey KopczynskiI.PREPARATION OF EMBRYOS1. Embryos were collected for 24 hours and washed 3X with 0.02% Triton X (Tx)(15ml for up to 2ml embryos in a 50ml falcon tube).2. Dechorionate 5 min. in 50% bleach.3. Wash 3X with 0.02% Tx.4. Fill tube
Replication timing by density transfer
for ~1 min. You can empirically determine how long it takes to bring your desired volume of medium from 37o to 23o C. 6. Collecting samples: Freeze 8 ml of 0.1% Na-azide, 0.2 M EDTA in "50 ml" Oakridge-type plastic, screw-capped centrifuge tubes
The single most important advance in the use of centrifugal force to separate biologically important substances. was the coupling of mechanics, optics and mathematics by T. Svedberg and J.W. Williams in the 1920's. They initiated the mathematics
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