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上海研谨生物科技有限公司
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文献和实验with the better the chances of a successful pulldown. A total of 4.0×106 293 HEK cells (~70% confluent) are plated and 24 hours later transfected with 15µg of a RNA Polymerase II expressing flag-tagged construct plasmid. We generally use Lipofectamine 2000™
Rapid Extraction of High Quality DNA from Whole Blood Stored at 4ºC for Long Period
or at refrigerator for later uses. Standard chemicals This method uses standard chemicals that can be obtained from any major supplier; we used chemicals supplied by Sigma Co. as follow: EDTA (0.5 M), pH 8.0: Add 186.1 gr of anhydrous EDTA to 800 ml
µCi/µl, specific activity 3000 Ci/mmol) (for hot-stop PCR [Steps 1-22] or SSCP [Steps 23-37] only)dNTPs (stock solutions at 25 mM for each dNTP)DNA loading buffer (6X)30% (v/v) glycerol0.25% (w/v) bromophenol blue0.25% (w/v) xylene cyanol FFStore
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