GLA 5000 PVC 圆盘过滤膜片，带衬垫，5 µm，3

Targeted Differential Display

below is one example of differential gene expression analysis of cells obtained from microdissected tissue. 1. Materials Stratagene RAP-PCR kit       5X RT buffer (GenHunter)       dNTP, 250 µM (GenHunter)       dNTP, 25 µM (GenHunter)       RNase inhibitor (Perkin

Detection and Analysis of Protein‐Protein Interactions of Organellar and Prokaryotic Proteomes by Blue Native and Colorless Native Gel Electrophoresis

or polypropylene, 40‐ to 50‐µm thick) Electrophoresis apparatus: Protean II 16‐cm cell (Bio‐Rad) or SE 600 16‐cm unit (Hoefer) with clamps, glass plates, casting stand, and buffer chambers 1.5‐mm

Methyltransferase-based single-promoter analysis assay

3). Samples are then centrifuged for 5 min at 5000 rpm at 4°C. The supernatant is discarded. The nuclei are then resuspended in 1X M.SssI buffer so that there are 106 nuclei per 74.25µl. M.SssI treatments M.SssI treatments of nuclei