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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20˚C/-80˚C
- 保质期:
24months
- 英文名:
Recombinant Putative defensin-like protein 70
- 库存:
详询
- 规格:
详询
Host Species:Yeast
Target Name:DEF70 Uniprot ID: P82792 Tag Info:GST-tag 产品详细说明请点击:Recombinant Putative defensin-like protein 70风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Expression and Purification of Recombinant -Defensins and -Defensin Precursors in Escherichia coli
Recombinant expression of α-defensins can be obtained at efficient levels in Escherichia coli. Amplified α-defensin or pro-α-defensin coding cDNA sequences are cloned directionally between EcoRI and SalI sites of the pET-28a expression vector
of proteins in both normal and stressed cells. The rational manipulation of chaperone levels in a cell line engineered to produce a defined recombinant protein (rP) can significantly improve both the achievable steady-state levels and authenticity of a wide
of scaffolding effects and ATP-driven conformational changes that mediate the temporary unfolding and subsequent refolding of protein substrates. DnaK and GroEL are the two major Escherichia coli chaperones. They belong to the HSP70 and HSP60 families of proteins
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