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- Signalway Antibody
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- ap72376
- 2025年10月21日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Store at -20˚C/-80˚C
- 保质期：
24months
- 英文名：
Recombinant Baker's yeast Neutral trehalase
- 库存：
详询
- 规格：
详询
Host Species:E.coli
Target Name:TREA Uniprot ID: P32356 Tag Info:His-tag 产品详细说明请点击：Recombinant Baker's yeast Neutral trehalase风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Yeast Transformation and the Preparation of Frozen Spheroplasts for Electroporation
Transformation methods for the baker’s yeast Saccharomyces cerevisiae have allowed for rapid advances in molecular genetic studies with this model microorganism, for the development of alternative expression systems to prepare recombinant
Alternative: Instead of recombinant lyticase, can also use Zymolyase 100T from ICN. Use ~2 mg per 2 liters of cells. The amount required can vary from 2-10 mg depending on the yeast strain. If using zymolyase, add in one extra YPD/S washing step
Yeast Nuclear Extract (Small Scale)
Yeast Nuclear Extract (Small Scale) Hahn Lab (adapted from J.Leatherwood,Ptashne lab) last modified Mon,Aug 27,2001 Cell Growth 3 liters of cells are grown in YPD (3% glucose)to A600 of 3-5.Antifoam A (two drops from a P200 pipetman)is added
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