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文献和实验A safe method of extracting DNA from Coccidioides immitis
nitrogen, lyophilized, and ground with a mortar and pestle. About 10 mg of dry ground tissue is mixed with 400 ul lysis buffer (50 mM Tris, 100 uM NaCl, 5 mM EDTA, 1 sodium dodecyl sulfate), and incubated at 80 C for 10 min., cooled to 40 C and incubated
A safe method of extracting DNA from Coccidioides immitis
extraction. Cultures are thawed, filtered, frozen in liquid nitrogen, lyophilized, and ground with a mortar and pestle. About 10 mg of dry ground tissue is mixed with 400 ul lysis buffer (50 mM Tris, 100 uM NaCl, 5 mM EDTA, 1% sodium dodecyl sulfate
RNA Purification Protocol for 1-2 x 107 cultured cells
设备 1. Microcentrifuge capable of 3,000 x g 2. 15 ml centrifuge tube 3. Ice bath 4. Ground pestle 实验步骤 1. Remove the media and collect cells. For cells grown in suspension, pellet the cell
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