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文献和实验Design of the Invader Genotyping Assay
, 47 bases PRIMER_PRODUCT_SIZE_RANGE=81-600 PRIMER_FIRST_BASE_INDEX=1 PRIMER_GC_CLAMP=1 PRIMER_OPT_SIZE=20 PRIMER_MAX_SIZE=25 PRIMER_MIN_SIZE=17 PRIMER_OPT_TM=60 PRIMER_MAX_TM=64 PRIMER_MIN_TM=58 PRIMER_MAX_DIFF=100 PRIMER_MIN_GC=30 PRIMER
A General Method for Quantifying Sequence Effects on Nucleobase Oxidation in DNA
proportions of both base and sugar damage. To this end, we have developed a universal method to quantify sequence context effects on nucleobase damage without interference by strand breaks from deoxyribose oxidation.
Taq-polymerase catalyzed cycle sequencing using fluorescent-labeled dye primers
Each base-specific fluorescent-labeled cycle sequencing reaction routinely included approximately 100 or 200 ng Biomek isolated single-stranded DNA for A and C or G and T reactions, respectively. Double-stranded cycle sequencing
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