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文献和实验; M. pirum) PCR reagents : Taq polymerase ( 5 U / ul ) dNTP( dGTP, dCTP, dTTP, dATP, 1.25 mM each ) 10x PCR buffer (with 1.5mM MgCl2) 25mM MgCl2 sterile ddH2O Machine / Equipment： PCR thermal cycler agarose电泳设备 DNA电泳胶体观察设备 无菌PCR反应管 无菌1.5 ml
was set to 50 μL/min, and the working temperature was fixed at 25°C.Different concentrations of hIgE prepared in a two-fold dilution in the running buffer (10 mM PBS + 1 mM MgCl2) were injected in order to determine the affinity between hIgE
Nucleobond Column BAC DNA Purification for Transgenic Mouse Production
, there are a number of factors that could adversely affect plasmid yield, in the case of low copy number plasmids, incomplete bacterial lysis is the number one culprit. The primary reason for this is that in order to get the maximum yield for a particular cartridge
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