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文献和实验CGH Image acquisition Images were acquired through a Zeiss Axiophot fluorescence microscope using a Plan NEOFLUAR oil objective x63, N.A. 1.25 (Zeiss, Oberkochen, Germany) equipped with filter sets appropriate for DAPI (Zeiss filter set
and 37°C, oxygenated by bubbling with carbogen gas (95% O 2 /5% CO 2 ) 0.1 M KCl stock solution Polyethylene cannula
RLCS (Restriction Landmark cDNA Scanning) Protocol
tube placed in a magnet stand (MPC-M). 3. After 30 sec., remove supernatant. Note: Do not leave Dynabeads Oligo(dT)25 unsuspended for a long time. 4. Remove tube from MPC-M and prewash Dynabeads Oligo(dT)25 by resuspending in 200 ul Lysis
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