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上海希言科学仪器有限公司
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文献和实验Silica Suspension: add 2 g of silica to 15 ml of H2O wash 3x by centifugation at 2000 x g for 2 min estimate vol of silica and resuspend in 2 vol H2O Silica Wash Solution: 50 mM NaCl,10 mM Tris 7.5,2.5 mM EDTA,50% Ethanol 6 M NaI note
DNA Extraction Protocols Using Silica
at room temperature).B. Extraction buffer1. Combine 24 g GuSCN (guanidinium thiocyanate) and 20 mL 0.1 M Tris-HCl (pH 6.4) in a 50 mL Falcon tube.2. Heat to 60 oC to dissolve the GuSCN.3. Add 4.4 mL of 0.2 M EDTA (pH 8.0).4. Add 0.5 mL Triton X-100.5. Mix
resuspend silica pellet in desired volume of 10 mM Tris 7.5 or TE or 1X restriction buffer for downstream digests heat 5 minutes at 60 degrees spin 2 min @ full speed in microcentrifuge remove DNA -containing supernatant to clean tube Notes & Misc
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