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- 2025年07月11日
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上海希言科学仪器有限公司
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文献和实验Clonality - X Chromosome Inactivation Assay
(1:1:1, v:v:v) (Gibco) 3 M Na Acetate, pH 5.2 (Life Technologies) Isopropanol Glycogen, 10 mg/ml (GenHunter) 70% ethanol Buffer 1 (50 mM Tris-HCl, pH 8.0, 10 mM MgCl2, 50 mM NaCl) Hha I restriction enzyme (Life Technologies) REact 2 Buffer
Method for Single-Cell Electroporation
-10 µm Eppindorf Pipettor tip, or a 1 cc plastic insulin syringe that had been melted over flame and pulled to a long fine tip. Circuit setup: A thin silver wire (diameter 0.25mm) is inserted into the micropipette touching the DNA solution
-HCL PH 6.6, and that is better) Buffer PE 10 mM Tris-HCl pH 7.5 80% ethanol Buffer QBT equilibration buffer 750 mM NaCl 50 mM MOPS pH 7.0 15% isopropanol 0.15% triton X-100 Buffer QC wash buffer
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