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文献和实验Purification of recombinant sBRF M166L
culture with 0.4 mM IPTG (4 ml of 0.1 M IPTG per 1000 ml) and grow at 30 deg. for four hours. Take a small sample of the induced and uninduced cultures and save for analysis of whole cell protein by SDS PAGE. Harvest cells by centrifugation (4-6g
Lacrimal Experimental Protocol (4/4/94)
bFGF (Sigma F9786): dexamethasone 30 µl of 10 µg/ml stock putrescine 300 µl of 100 mM stock [l-ascorbic acid 60 µl of 12.5 mg/ml stock]* ITS 360 µl of 1.25 mg/ml; 1.25 µg/ml stock glutathione 300 µl of 1 mg/ml stock EGF 30 µl of 50 µg/ml stock HEPES 456
Purification of recombinant sBRF M166L
with 0.4 mM IPTG (4 ml of 0.1 M IPTG per 1000 ml)and grow at 30 deg.for four hours.Take a small sample of the induced and uninduced cultures and save for analysis of whole cell protein by SDS PAGE. Harvest cells by centrifugation (4-6g).Resuspend the cell
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