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文献和实验Construction and Manipulation of Large-Insert Bacterial Clone Libraries
. II. Reagents 10 x homogenization buffer (HB) stock: 0.1 M Trizma base, 0.8 M KCl, 0.1 M EDTA, 10 mM
/ml 硝酸, 10. 20 µg/ml 4-羟基苯甲酸 乙酸及氟代乙酸的分离 Conditions: column: Dionex Acclaim 120 C18 5 m 120 Å (4.6 x 100 mm, ID coated first with 5 mM Triton X-100 and then 5 mM CPC; eluent: 10 mM Na2CO3; flow rate, 1.0 ml/min; detection
-stop PCR only; see Steps 1-22)SSCP loading dye (for SSCP only; see Steps 23-37)95% (v/v) formamide10 mM NaOH0.25% (w/v) bromophenol blue0.25% (w/v) xylene cyanolTaq DNA polymerase (5 U/µl)TBE Buffer (1X and 5X)Prepare a 5x stock solution in 1 liter
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