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上海希言科学仪器有限公司
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文献和实验.27. Column Buffers Make 500mls equivalent of base buffer: 10ml 1M Hepes pH 7.9 100ml 10% glycerol 5ml 10% NP40 (or 5.0ml 300mM Octyl glucoside) 200ml 0.5 EDTA ------- 115.2ml /5 = 23ml Base 1M KCl2.5M KCl dH20 for 0 KCL 46ml 0 0 154ml = 200
elution buffer combine supernatants, incubate at 65 °6 h to ON take 1/100 of the protein amount taken for the IP, add to 150 µl elution buffer (INPUT control) incubate at 65 °C 6 h to ON add 750 µl PB buffer (Qiagen
/ml 硝酸, 10. 20 µg/ml 4-羟基苯甲酸 乙酸及氟代乙酸的分离 Conditions: column: Dionex Acclaim 120 C18 5 m 120 Å (4.6 x 100 mm, ID coated first with 5 mM Triton X-100 and then 5 mM CPC; eluent: 10 mM Na2CO3; flow rate, 1.0 ml/min; detection
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