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上海希言科学仪器有限公司
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文献和实验ChIP protocol for X. laevis Lens1/FoxE3 enhancer
at �80°C. (14) Aliquot 100 µl of the supernatants into 1.5 ml microfuge tubes, and add 900 µl of 150 mM NaCl ChIP Dilution buffer/1x proteinase inhibitors. This dilution decreases the SDS concentration in chromatin samples
/ml 硝酸, 10. 20 µg/ml 4-羟基苯甲酸 乙酸及氟代乙酸的分离 Conditions: column: Dionex Acclaim 120 C18 5 m 120 Å (4.6 x 100 mm, ID coated first with 5 mM Triton X-100 and then 5 mM CPC; eluent: 10 mM Na2CO3; flow rate, 1.0 ml/min; detection
elution buffer combine supernatants, incubate at 65 °6 h to ON take 1/100 of the protein amount taken for the IP, add to 150 µl elution buffer (INPUT control) incubate at 65 °C 6 h to ON add 750 µl PB buffer (Qiagen
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