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- 2025年07月09日
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文献和实验A quick RNA mini-prep for Neurospora mycelial cultures
Most RNA isolation techniques currently in use have been developed for the processing of large quantities of material. These typically involve multiple phenol extractions (Reinert et al. 1981 Mol. Cell Biol. 1:829-836) or guanadinium
and thereby ensure maximal growth and plasmid DNA yields). 2nd Day -DNA extraction and purification Materials required QIAGEN Mini-prep kit Microfuge Cell Lysis/DNA Extraction 1. Concentrate the bacteria by transferring 1 ml bacterial suspension
Fast and reliable mini-prep RNA extraction from Neurospora crassa
the method we modified the protocol used for Chlamydomonas by Gromoff et al. (Mol. Cell. Biol. 1989. 9:3911-3918) who combined the advantages of phenol extraction and LiCl precipitation. The procedure is suitable for rapidly grown mycelia, starved mycelia
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