- 询价
- 美国Corning Falcon
- 美国
- 352090
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
上海希言科学仪器有限公司
- 规格：
8个/箱
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文献和实验BAC DNA Isolation from 200 ml Cultures by a Cleared Lysate Method
7.6, 50 mM EDTA, pH 8.0), and 1.8 ml of 7.5 M KOAc (note: the 7.5 M KOAc solution is used without pH adjustment) is added into each bottle and transfered to a 50 ml Sorval centrifuge tube. 8、After mixing, the tubes were frozen, until solid, at -70
for 5 minutes in 50 ml conical tubes in the Beckman GPR tabletop centrifuge and decant the supernatant. The cell pellets can be frozen at -70degC at this point. 3. Resuspend the cell pellets in 2 ml of GET/Lysozyme solution, add 4 ml of alkaline lysis
flask 1-2 times per day Add Colcemid (about 45 min before harvesting) Make 2 aliquots and transfer cell into 50 ml Falcon tubes Incubate in cell cuture incubator or 37°C water bath for additional 45 min Centrifuge for 10 min
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