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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件:
冷藏
- 保质期:
长期
- 库存:
大量
- 供应商:
康朗生物
- 规格:
2ml
Protein A+G Agarose
规格:2ml产品介绍:
本产品为Protein A和Protein G 共价交联到4% Agarose beads上的产物,并保存在含有0.05%叠氮钠的TBS缓冲液中,2 ml Protein A+G Agarose中共含有0.5 ml Agarose beads,约2 mg重组的Protein A+G。主要用于免疫沉(Immunoprecipitation,IP)或免疫共沉淀(Co-IP),也可以用于抗体的纯化。适用于免疫沉淀所有Protein A Agarose和Protein G Agarose单独可以免疫沉淀的抗体,包括Mouse IgG1,IgG2a,IgG2b,IgG3,IgA,Rat IgG1,IgG2a,IgG2b,IgG2c,Rabbit IgG,Rabbit and Goat polyclonal Abs,以及Human IgG1,IgG2,IgG3和IgG4。本产品如果用于常规的免疫沉淀,可以免疫沉淀100次。
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文献和实验ANTIBODY BINDING TO PROTEIN A AND PROTEIN G
Protein A, derived from the cell wall of Staphylococcus aureus , and protein G, derived from the cell wall of certain strains of b -hemolytic Streptococcci , binds the Fc region
Antibody Purification: Affinity Chromatography Protein A and Protein G Sepharose
G coupled to agarose. In the Subheading 3 there are also protocols for affinity purification using a specific ligand coupled to commercial matrices like CNBr- Sepharose 4-B and Affigel.
10 mg/ml ethidium bromide To prepare 100 ml of a 0.7% agarose solution, measure 0.7 g agarose into a glass beaker or flask and add 100 ml 1X TBE or TAE. Microwave or stir on a hot plate until agarose is dissolved
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