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文献和实验Macrophage Depletion by Clodronate
through the Bilbate Ж pipette placed on the bottom of the tube for 60-90 min, RT. centrifuge for 15 min at 10000 rpm (rotor ID=JA20), 24ѓC aspirate the solution under the liposomes'' ring (in case of liposome-clodronate) or aspirate the supernatant (SN
(known as a cytofectin; cyto for cell and fectin for transfection [i.e., gene delivery and expression]) or, more commonly, from the combination of a cytofectin and a neutral lipid such as dioleoyl l -α-phosphatidylethanolamine (DOPE) or cholesterol (Chol
Liposomes for Specific Depletion of Macrophages from Organs and Tissues
as the liposomes are digested with the help of its lysosomal phospholipases. At a certain intracellular clodronate concentration, the macrophage is eliminated by apoptosis. Given the fact that, neither the liposomal phospholipids chosen, nor clodronate are toxic
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