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文献和实验Aspects of CNS Lupus: Mouse Models of Anti-NMDA Receptor Antibody Mediated Reactivity
concepts of these models and provide protocols for the following: (1) the induction of anti-dsDNA, anti-NMDAR antibodies, (2) testing serum antibody titer by ELISA, (3) breaching blood brain barrier (BBB) integrity with LPS and epinephrine, (4) passive
This work describes protocols for the production of single-chain antibody and T-cell receptor fragments inE. coli . A choice of methods is given for the purification of the recombinant fragments that rely on the use of either immunoaffinity
Antibody Targeted siRNA Delivery
only. The commercial success of in vivo applications of RNAi hinges on the development of new delivery methods. Our strategy involves the use of antibody-based delivery agents to target and deliver siRNA into specific cell types. We have developed antibody-based agents
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