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文献和实验秦奋 为什么HIV-gp41与原核PET28a质粒构建后,行诱导表达,SDS-PAGE无结果,而WB却有结果,这种情况怎么回事?(构建后测序同源性达95%,而且已酶切认证构建成功) 谢谢 zhujoker “SDS-PAGE无结果,而WB却有结果”是什么意思,你表述清楚一点,要不人家会有疑惑的。其实这主要看你是否诱导成功。个人认为还有就是你在原核里面表达,作为包涵体存在形式,你是否将蛋白提出来了呢?
Assessment of Compounds for Anti-HIV Activity
of the receptor for the virus (1 ). The basic principle when testing for anti-HIV compounds involves the interaction of virus with test material under suitable culture conditions and the subsequent measurement of a parameter reflecting the amount of virus present
In Vivo Testing of Anti-HIV Immunotoxins
The past several years have seen great optimism resulting from the deployment of highly active antiretroviral therapies for the treatment of HIV infection. Combinations of reverse transcriptase and protease inhibitors can induce suppression
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