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- American Research Products
- 06-1064-2-50 ug
- 2025年07月30日
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50 ug
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文献和实验Myosin Light Chain Preparation
in lyophilizer bottles and freeze in liquid nitrogen.Put on lyophilizer and leave until dry (overnight or over weekend). Day 5 22.Resuspend separate light chain pools 1 and 2 in a small volume of Dialyzing Solution G.Pipette into dialyzing tubing (size #20
Purification of Myosin Light Chain Kinase
RPM (4℃).Filter the supernatant through glass wool and discard the pellet (or save for light chain prep).Save 1 ml of supernatant (4℃)for assay.Add pepstatin and TLCK to final concentrations of 1 μg/ml and 40 Og/ml,respectively. Volume of supernatant
(2 ). Natural autoantibodies crossreact with a variety of conserved, and seemingly unrelated, autoantigens. Most of these antibodies are of low affinity, although rare exceptions of high affinity natural anti-DNA antibodies have been reported (3 ). The role
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