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文献和实验that for several proteins, the first ubiquitin moiety is fused linearly to the α-NH2 group of the N-terminal residue. Important biological questions relate (1) to the evolutionary requirement for an alternative mode of ubiquitination, (2) to the identity of the set
N-Terminal Amino Acid Sequencing of 2-DE Spots
Two-dimensional electrophoresis (2-DE) is one of the most advanced techniques for direct separation of considerable numbers of proteins. Direct microsequence analysis of the amino (N)-terminal partial sequence of the electroblotted protein
The Use of TAGZyme for the Efficient Removal of N-Terminal His-Tags
is unwanted or may represent a disadvantage for the projected use of the protein, like in clinical, functional or structural studies. For N-terminal tags, the TAGZyme system represents an ideal approach for fast and accurate tag removal. TAGZyme is based
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