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- American Research Products
- 01-P2684-0.5 mg
- 2025年07月12日
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文献和实验Purification of Human Multiprotein Complexes using OneSTrEP Technology
. For more theoretical background and a protocol for purification of recombinant Strep -tag® fusion proteins from E.coli please refer to Schmidt and Skerra (2007). In comparison to other commonly used purification strategies (i.e. HA and FLAG double-tag
Purification of Human Multiprotein Complexes using OneSTrEP Technology (PROT41)
complexes from human HeLa S3 cells in a scale and purity optimized for characterization by mass spectrometry. For this purpose, we use stably expressed One-STrEP -tag ® fusion proteins. This approach was successfully used in characterization of histone
隆抗体HIT3a和PHMA02轻、重链可变区基因的基础上，构建和表达了抗CD3/抗Pgp Diabody，并研究了其生物学功能。一、材料与方法1、材料（1）细胞系和载体E.coli 16C9由本室保存。多药耐药细胞系K562/A02由本室建立[7]。Jurkat 和K562细胞系有本室保存，培养在含10%小牛血清（本所科技开发公司）的RPMI1640（GIBCOBRL）。抗CD3 ScFv表达载体[8]和抗Pgp ScFv表达载体本室构建。表达载体pYZF和克隆载体pYZL由我室保存。（2）酶和试剂Tag
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