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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Synthetic peptide within Human CD63
- 亚型:
IgG
- 形态:
液体
- 保存条件:
+4°C 下短期储存,-20°C 下长期储存。避免冻融循环。
- 克隆性:
多克隆
- 适应物种:
人,鼠
- 库存:
大量
- 供应商:
北京百奥创新科技有限公司
- 宿主:
兔
- 应用范围:
IHC-P, WB
- 抗体英文名:
Anti-CD63 antibody - Late Endosome Marker
- 抗体名:
Anti-CD63 antibody - Late Endosome Marker
- 规格:
100ul
产品货号:Ab216130
产品规格:100ul
保存条件:+4°C 下短期储存,-20°C 下长期储存。避免冻融循环。
形态:液体
应用范围:IHC-P, WB
保质期:按说明书条件可保存1-2年
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD63 antibody - Late Endosome Marker (ab216130)
Paraffin-embedded human liver cancer tissue stained for CD63 with ab216130 at a 1/25 dilution in immunohistochemical analysis.
Heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.
All lanes : Anti-CD63 antibody - Late Endosome Marker (ab216130) at 1/200 dilution
Lane 1 : NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate
Lane 2 : K562 (Human chronic myelogenous leukemia cell line from bone marrow cell lysate
Lane 3 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lysates/proteins at 40 µg per lane.
Developed using the ECL technique.
Predicted band size: 26 kDa
Exposure time: 20 seconds
Reducing 12% SDS-PAGE
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using an anti-4-HNE monoclonal antibody (MAb) (6 –8 ) and labeled goat anti-mouse IgG antibody (9 ).
祝贺〖雅裕睿安〗成为Signalway Antibody(SAB)独家
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The production of monoclonal antibody by hybridoma
The production of monoclonal antibody by hybridoma fusion: Immortalization of sensitized B lymphocytes from immune mice. Overview
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