![HRP Anti-beta Actin antibody [AC-15]](https://img1.dxycdn.com/2023/0601/317/6775343473947804661-14.jpg)
- ￥3800 - 4300
- Abcam
- 英国
- Ab49900
- 2025年07月13日
- WB
- 鼠
- 人、鼠
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Synthetic peptide corresponding to beta Actin (N terminal) conjugated to keyhole limpet haemocyanin.
- 亚型：
IgG1
- 形态：
液体
- 保存条件：
+4°C 下储存
- 克隆性：
单克隆
- 适应物种：
人、鼠
- 库存：
大量
- 供应商：
北京百奥创新科技有限公司
- 宿主：
鼠
- 应用范围：
WB
- 抗体英文名：
HRP Anti-beta Actin antibody [AC-15]
- 抗体名：
HRP Anti-beta Actin antibody [AC-15]
- 规格：
50ul
产品货号：Ab49900
产品规格：50ul
保存条件：保存于-20℃
形态：液体
应用范围：WB
保质期：按说明书条件可保存1-2年
Proteins were loaded onto a 10% SDS-polyacrylamide gel. After gel electrophoresis, blots were subsequently probed with primary antibodies anti- IL-6 at 1:1000 , anti- IL-17 at 1:3000, anti-TGF-β at 1:1000. For detection, horseradish peroxidase-conjugated secondary anti-rabbit antibody was used followed by enhanced chemiluminescence development.
Normalization of results was ensured by running parallel western blots with β-actin antibody at 1:25,000 (ab49900). The optical density was quantified using an image densitometer. The data are presented as a percentage of target protein relative to β-actin. A value of p<0.05 is considered significant.
Western blot - HRP Anti-beta Actin antibody [AC-15] (ab49900)
All lanes : HRP Anti-beta Actin antibody [AC-15] (ab49900) at 1/50000 dilution
Lane 1 : A549 Lysate
Lane 2 : HeLa Lysate
Lane 3 : 3T3 Lysate
Lane 4 : NRK Lysate
Lane 5 : MDCK Lysate
Lane 6 : MDBK Lysate
Lane 7 : BHK Lysate
Lane 8 : COS7 Lysate
Lane 9 : CFB Lysate
Developed using the ECL technique.
Predicted band size: 42 kDa
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文献和实验Bechara R et al. The RNA-binding protein IMP2 drives a stromal-Th17 cell circuit in autoimmune neuroinflammation. JCI Insight 7:N/A (2022).
技术资料