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- 技术资料
- 英文名：
Agilent DNA 1000 Kit
- 库存：
现货
- 供应商：
广州柏赛柯生物
DNA Analysis Kits
客服热线：020-39341005
联系客服QQ: 1195082744
The DNA kits are ideal for automated sizing and quantitation of PCR fragments, restriction digests or fragmented DNA.
- Broad linear dynamic range –detection of less abundant products, e.g. non-specific amplifications
- High resolution – separate fragments down to 5 bp
- Sizing accuracy – normalization to internal markers and ladder
- Quantitation accuracy and reproducibility – quantitation of each DNA fragment against internal standards
- Minimal sample consumption – only 1 µL sample required
The DNA 1000, 7500 and 12000 kits offer automated analysis of PCR fragments, restriction digests and
next-generation sequencing (NGS) samples. Typical application areas are: amplicon QC in
forensic DNA studies, gene expression analysis by RT-PCR, DNA QC for NGS, or detection
of genetically modified organisms.
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The DNA1000 kit outperforms traditional slab gels when analyzing a mixture of 13multiplex PCR products with its high resolution and the reliable sizing and quantitation of each individualDNA fragment. (Application note 5988-9342EN) |
| Analytical specifications | DNA 1000 Kit | DNA 7500 Kit | DNA 12000 Kit |
| Sizing range | 25–1000 bp | 100–7500 bp | 100–12000 bp |
| Sizing resolution | 25–100 bp: 5 bp 100–500 bp: 5% 500–1000 bp: 10% |
100–1000 bp: 5% 1000–7500 bp: 15% |
100–1000 bp: 5% 1000–12000 bp: 15% |
| Sizing accuracy | ± 10% | ± 10% | ± 15% |
| Sizing reproducibility | 5% CV | 5% CV | 5% CV |
| Quantitation accuracy1 | 20% | 20% | 25% |
| Quantitation reproducibility1 | 25–500 bp:15% CV 500-1000 bp:5% CV |
100-1000 bp:10% CV 1000-7500 bp: 5% CV |
100-1000 bp: 15% CV 1000-12000 bp: 10% CV |
| Quantitative range1 | 0.5-50 ng/µL | 0.5-50 ng/µL | 0.5-50 ng/µL |
| Maximum salt concentration in sample | 250 mM for KCl, 15 mM for MgCl2, 250 mM NaCl |
250 mM for KCl, 15 mM for MgCl2, 250 mM NaCl |
250 mM for KCl, 15 mM for MgCl2, 250 mM NaCl |
| Physical specifications | |||
| Analysis time | 35 minutes | 30 minutes | 30 minutes |
| Samples per chip | 12 | 12 | 12 |
| Sample volume | 1 µL | 1 µL | 1 µL |
| Kit stability | 4 months (see box for storage temperatures) | 4 months (see box for storage temperatures) | 4 months (see box for storage temperatures) |
| Kit size | 25 chips 12 samples/chip = 300 samples/kit |
25 chips 12 samples/chip = 300 samples/kit |
25 chips 12 samples/chip = 300 samples/kit |
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文献和实验相关实验
RNA was purified using the AllPrep DNA/RNA 96 Kit and analyzed on the Agilent 2100 bioanalyzer. Intact RNA is indicated by a RIN value of close to 10. RNA with no genomic DNA contamination. Total RNA was purified from rat kidney
实验试剂 1. Sterile deionized water (or TE buffer) 2. Absolute (or 95%) ethanol 实验设备 1. Sterile 1.5 mL centrifuge tubes. 2. Protective eye-ware 实验步骤 1. MicroElute™ DNA
for 2 mL tube to magnetize the MagSi particles. Carefully remove and discard the cleared supernatant. 11. Remove the tube containing the MagSi? particles from the magnetic separation device. 12. Add 1000 ul Buffer PHB into the tube
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Agilent DNA 1000 Kit
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