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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
低温
- 保质期:
2年
- 英文名:
Recombinant Human Cardiotrophin-1 (CT-1) Protein, CF
- 库存:
大量
- 供应商:
北京百奥创新科技有限公司
- 规格:
具体价格请联系我们
Purity:>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level:<0.01 EU per 1 μg of the protein by the LAL method.
Activity:Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 0.25‑1.25 ng/mL.
Source:E. coli-derived human Cardiotrophin-1/CT-1 protein
Ser2-Ala201
N-terminal Sequence Analysis:Ser2
Predicted Molecular Mass:21 kDa

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文献和实验北京百奥创新
High Throughput Production of Recombinant Human Proteins for Crystallography
This chapter presents in detail the process used in high throughput bacterial production of recombinant human proteins for crystal structure determination. The core principles are: (1 ) Generating at least 10 truncated constructs
Experimental Applications: Human Acetylcholinesterase as a Model Nervous System Protein
, 5 ng in vitro-transcribed AChE mRNA directed the production of catalytically active recombinant human acetylcholinesterase (rHAChE), at levels of approx 10-fold above background oocyte levels (Table 6 ). Oocyte-produced rHAChE was sensitive to the AChE
followed by reinfection of E. coli cells with the eluted bound phages ( see refs. 1 – 10 ). An outline of the method is illustrated in Fig. 1 . Fig. 1. Overview of the steps involved in the generation of human antibody Fab libraries
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