手机验证
-20℃
一年
STAT3 Mutation Probe For EMSA(10μM)
264
北京百奥莱博科技有限公司
30μl
特别声明:本产品及我公司所售其他产品均为科研类试剂产品,严禁用于药物、医疗及其他非科研用途。
北京百奥莱博专业生产销售YT257型STAT3凝胶迁移突变探针(10μM)(国产,进口),我公司供应的细胞生物学试剂品类齐全,且具有优势价格,欢迎新老客户垂询订购。
名称:YT257型STAT3凝胶迁移突变探针(10μM)(国产,进口)
品牌:百奥莱博
英文名:STAT3 Mutation Probe For EMSA(10μM)
产地:国产|进口
编号:YT257
本探针是用于EMSA(也称gel shift)研究的STAT3 consensus oligonucleotide的突变体。可以作为EMSA探针-STAT3的阴性对照,用于EMSA结合反应中突变探针的冷竞争反应等。一个包装的突变探针,如果用于同位素标记EMSA探针的突变探针冷竞争反应,可以进行90-180个突变探针的冷竞争反应。如果用于生物素标记探针的冷竞争时,可以进行约30个冷竞争反应。
STAT3 consensus oligo的序列如下:
5"-GAT CCT TCT GGG AAT TCC TAG ATC-3"
3"-CTA GGA AGA CCC TTA AGG ATC TAG-5"
EMSA突变探针-STAT3中STAT3的公认的结合位点发生了突变,从而使STAT3无法和该突变探针结合。在探针冷竞争反应中,正常的标记探针和STAT3的结合的条带会被抑制;而在突变探针冷竞争反应(cold competition)中,正常的标记探针和STAT3的结合的条带不会被抑制。参考下图。
一个典型的EMSA/Gel- Shift分析图
1,阴性对照反应(标记探针);
2,常规反应(含激活的目的转录因子的核蛋白+标记探针);
3,探针冷竞争反应(含激活的目的转录因子的核蛋白+标记探针+标记探针100倍量的未标记探针);
4,突变探针的冷竞争反应(含激活的目的转录因子的核蛋白+标记探针+标记探针100倍量的未标记突变探针);
5,Super-shift反应(含激活的目的转录因子的核蛋白+标记探针+目的转录因子的特异抗体)。
注意事项:
1. 避免加热到40℃以上,温度过高会导致双链DNA探针解聚成单链。而单链无法用于EMSA研究。
2. 对于EMSA的详细操作可以参考我们的EMSA试剂盒的使用说明。
储存条件:-20℃,有效期一年。
除YT257型STAT3凝胶迁移突变探针(10μM)(国产,进口)外,我公司正在打折促销以下产品:
·兔抗IDH1抗体(异柠檬酸脱氢酶抗体)
编号:YT734
英文名称:Isocitrate dehydrogenase/IDH1 Mouse Monoclonal Antibody
规格:50μl
本品为来源于小鼠的抗异柠檬酸脱氢酶(IDH1)的单克隆抗体。
宿主:小鼠
克隆性:单克隆抗体
用途:WB,IHC
交叉反应性:人
分子量:47KDa
推荐稀释比例:WB(1:1000),IHC(1:100-1:200)
About this Antibody | |
Name | Isocitrate dehydrogenase/IDH1 Mouse Monoclonal Antibody |
Category | Monoclonal antibody(mAb); Primary antibody |
Isotype | IgG1 |
Purification | ProA affinity purified |
About the Immunogen | |
Immunogen | This antibody is produced by immunizing mice with a recombinant protein corresponding to a region of IDH1. |
Gene ID | 3417 |
SwissProt | O75874 |
Synonyms | IDCD; IDH; IDP; IDPC; PICD |
Category | Metabolism |
Background | Isocitrate dehydrogenase (IDH) is an enzyme that catalyzes the oxidative decarboxylation of isocitrate, producing alpha-ketoglutarate (α-ketoglutarate) and CO2. In humans, IDH exists in three isoforms: IDH3 catalyzes the third step of the citric acid cycle while converting NAD+ to NADH in the mitochondria. The isoforms IDH1 and IDH2 catalyze the same reaction outside the context of the citric acid cycle and use NADP+ as a cofactor instead of NAD+. They localize to the cytosol as well as the mitochondrion and peroxisome. Mutations in IDH1 are also implicated in cancer. Originally mutations in IDH1 were detected in an integrated genomic analysis of human glioblastoma multiforme. In addition to being mutated in diffuse gliomas, IDH1 has also been shown to harbor mutations in human acute myeloid leukemia (AML). |
About this Antibody | |
Name | GRB2 Rabbit Polyclonal Antibody |
Category | Polyclonal antibody(pAb); Primary antibody |
Isotype | IgG |
Purification | Peptide affinity purified |
About the Immunogen | |
Immunogen | This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to adapter protein GRB2. |
Gene ID | 2885 |
SwissProt | P62993 |
Synonyms | ASH; Grb3-3; MST084; NCKAP2; MSTP084; EGFRBP-GRB2 |
Category | MAP Kinase Signaling |
Background | Grb2 is widely expressed and is essential for multiple cellular functions. Inhibition of Grb2 function impairs developmental processes in various organisms and blocks transformation and proliferation of various cell types, and so it is not surprising that a targeted gene disruption of Grb2 in mouse is lethal at an early embryonic stage. Grb2 is best known for its ability to link the epidermal growth factor receptor tyrosine kinase to the activation of Ras and its downstream kinases, ERK1,2. Grb2 is composed of an SH2 domain flanked on each side by an SH3 domain. Grb2 has two closely related proteins with similar domain organizations, Gads and Grap. Gads and Grap are expressed specifically in hematopoietic cells and function in the coordination of tyrosine kinase mediated signal transduction. The SH2 domain of Grb2 binds to phosphorylated tyrosine-containing peptides on receptors or scaffold proteins with a preference for pY-X-N-X, where X is generally a hydrophobic residue such as valine. |
我公司正在优惠促销凝胶迁移EMSA等系列产品,期待您的咨询选购YT257型STAT3凝胶迁移突变探针(10μM)(国产,进口)。
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