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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 保质期:
长期
- 英文名:
Ribonuclease A(DNase free)
- 库存:
大量
- 供应商:
康朗生物
- 规格:
0.5ml
RNase A (100mg/ml)(不含DNase)
规格:0.5ml编号:KL006
英文名:Ribonuclease A(DNase free)
本品用于消化RNA,浓度为10mg/ml,不含DNase,可直接使用。用于各种常见的分子生物学、细胞生物学等相关实验。
储存条件:-20℃。
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文献和实验Ribonuclease A (RNase A), DNase-free
guanidinium thiocyanate plus 0.1 M 2mercaptoethanol and heavy metal ions.Inactivated by phenol/chloroform extraction.Note The working concentration for RNase A is 1-100 µg/ml depending on the application. The enzyme is active under a wide range of reaction
Preparation of RNase-Free DNase by Alkylation
Characterization of RNA molecules by electrophoresis or hybridization frequently requires nucleic acid concentrations over 1 mg/mL. High molecular weight DNA in a mixture of nucleic acids limits the solubility and interferes
assay) 0.5μl H2O 3)DNAse mix: made up near end of binding incubations. DNAse l(Worthington DPFF,Cat#LS0006330, lot #58A047,5mg) is 1mg/ml in150mM NaCl, 50% glycerol, store at ©20℃. Try 3 different [s] ofDNAse mix (A,B,C) 1,2 & 3μl stock DNAse1
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