Liquid. Whole antiserum from rabbit containing 0.05% azide.
规格:
100 ul
NCBI Gene Id: 75605;
Swissprot Id: Q9UGL1
JARID1B (UniProt/Swiss-Prot entry Q9UGL1) is a histone demethylase which specifically demethylates mono-, di, and trimethylated lysine 4 of histone H3. It acts as a transcriptional repressor for the tumor suppressor genes BRCA1 and HOXA5 and has been associated with breast cancer development. In contrast, in melanoma JARID1B itself may function as a tumor suppressor. - See more at: http://www.avivasysbio.com/sd/tds/html_datasheet.php?sku=OADC00154
Mouse Jarid1b
Determination of the titer. To determine the titer, an ELISA was performed using a serial dilution of the Aviva antibody directed against mouse Jarid1b. The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:2,200.
Mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a)
Western blot analysis using the Aviva antibody directed against Jarid1b (1)r. Western blot was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) with the Aviva antibody against mouse Jarid1b, diluted 1:500 in BSA/ PBS-Tween. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest (expected size: 179 kDa) is indicated on the right.