Add 0.2ml of distilled water will yield a concentration of 500ug/ml. Store at -20C for one year. After reconstitution, at 4C for one month. It can also be aliquotted and stored frozen at -20C for a longer time. Avoid repeated freezing and thawing.
E.coli-derived human Dicer recombinant protein (Position: M1-N195). Human Dicer shares 94% amino acid (aa) sequence identity with mouse Dicer.
规格:
100 ug
NCBI Gene Id: 23405
Swissprot Id: Q9UPY3
Dicer(DICER1), also known as endoribonuclease Dicer or helicase with RNase motif, is an enzyme that in humans is encoded by the DICER1 gene. It is mapped to 14q32.13. The DICER1 gene, a member of the ribonuclease III (RNaseIII) family, is involved in the generation of microRNAs (miRNAs), which modulate gene expression at the posttranscriptional level. DICER1 possesses an RNA helicase motif containing a DEXH box in its amino terminus and an RNA motif in the carboxy terminus DICER, also known as helicase-MOI, is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small RNA component that represses gene expression. In addition, DICER1 is required for formation of the RNA induced silencing complex (RISC). It also cleaves double-stranded RNA to produce short interfering RNAs (siRNAs) which target the selective destruction of complementary RNAs. - See more at: http://www.avivasysbio.com/sd/tds/html_datasheet.php?sku=OABB00929
Human Mammary Cancer Tissue
Anti- Dicer antibody, OABB00929, IHC(P) IHC(P): Human Mammary Cancer Tissue
Hela Whole Cell Lysate, MCF-7 Whole Cell Lysate
Anti- Dicer antibody, OABB00929, Western blotting All lanes: Anti Dicer (OABB00929) at 0.5ug/ml Lane 1: Hela Whole Cell Lysate at 40ug Lane 2: MCF-7 Whole Cell Lysate at 40ug Predicted bind size: 217 kDa Observed bind size: 217 kDa
Recombinant Human Dicer Protein
Anti- Dicer antibody, OABB00929, Western blotting All lanes: Anti Dicer (OABB00929) at 0.5ug/ml WB: Recombinant Human Dicer Protein 0.5ng Predicted bind size: 39 kDa Observed bind size: 39 kDa