Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
形态:
Liquid. 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
亚型:
IgG
免疫原:
Recombinant protein encompassing a sequence within the center region of human Calpain 1. The exact sequence is proprietary.
规格:
100 ul
NCBI Gene Id: 823
Swissprot Id: P07384
The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. Several transcript variants encoding two different isoforms have been found for this gene. - See more at: http://www.avivasysbio.com/sd/tds/html_datasheet.php?sku=OAGA01891
Methanol-fixed A431
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed A431, using Calpain 1 (OAGA01891) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled (Red) at 1:2000.
Paraffin-embedded PC13 xenograft
Immunohistochemical analysis of paraffin-embedded PC13 xenograft, using Calpain 1 (OAGA01891) antibody at 1:500 dilution.
Jurkat
Sample (30 ug of whole cell lysate) A: Jurkat 7.5% SDS PAGE OAGA01891 diluted at 1:1000