All lanes: Anti-NFKBIE Antibody (N-term) at 1: 2000 dilution Lane 1: PC-3 whole cell lysates Lane 2: A375 whole cell lysates Lane 3: Hela whole cell lysates Lane 4: LNCaP whole cell lysates Lane 5: THP-1 whole cell lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size: 53 kDa Blocking/Dilution buffer: 5% NFDM/TBST.