Hela whole cell lysates, human skeletal muscle lysates
All lanes: Anti-ACTN2 Antibody at 1: 1000 dilution Lane 1: Hela whole cell lysates Lane 2: human skeletal muscle lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size: 104 kDa Blocking/Dilution buffer: 5% NFDM/TBST.