Maintain refrigerated at 2-8C for up to 6 months. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
形态:
Liquid. Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
亚型:
Rabbit Ig
规格:
400 ul
NCBI Gene Id: 6790
Swissprot Id: O14965
Chromosomal segregation during mitosis as well as meiosis is regulated by kinases and phosphatases. The Aurora kinases, members of the Ser/Thr protein kinase family, associate with microtubules during chromosome movement and segregation. Auroria kinase A may play a role in cell cycle regulation during anaphase and/or telophase, in relation to the function of the centrosome/spindle pole region during chromosome segregation. It may be involved in microtubule formation and/or stabilization. This protein has also been postulated to play a key role during tumor development and progression. Aurora kinase A localizes on centrosomes in interphase cells and at each spindle pole in mitosis. It is highly expressed in testis, weakly in skeletal muscle, thymus and spleen, and also highly expressed in colon, ovarian, prostate, neuroblastoma, breast and cervical cancer cell lines. Expression is cell-cycle regulated, low in G1/S, accumulates during G2/M, and decreases rapidly afterward. Defects in Aurora kinase A are responsible for numerical centrosome aberrations including aneuploidy. - See more at: http://www.avivasysbio.com/sd/tds/html_datasheet.php?sku=OAAB18594
Hela cells
Aurora-A Antibody (C-term) (Cat. #OAAB18594) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. Dilution: 1:10~50
Hela cell
Confocal immunofluorescent analysis of Aurora-A Antibody (C-term) (Cat#OAAB18594) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue). Dilution: 1:10~50
A2058 cell line lysate
Western blot analysis of anti-Aurora-A Pab (Cat. #OAAB18594) in A2058 cell line lysate. Aurora-A (arrow) was detected using the purified Pab. Dilution: 1:1000
293 cell lysates
Western blot analysis of Aurora-A (arrow) using rabbit polyclonal Aurora-A Antibody (C-term) (Cat. #OAAB18594). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the Aurora-A gene (Lane 2) (Origene Technologies). Dilution: 1:1000