The antibodies are stable for 12 months from date of receipt when stored at -20C to -70C. The antibodies can be stored at 2C to 8C for three month without detectable loss of activity. Avoid repeated freezing thawing cycles.
亚型:
Rabbit IgG
免疫原:
A synthetic peptide containing the motif (GATMKTFCG) without phosphorylation at Thr308 of Human AKT1.
规格:
100 ug
NCBI Gene Id: 207
Swissprot Id: P31749
Akt [also known as PKB (Protein kinase B) is a family of serine/threonine kinases that plays an important role in signal transduction. There are three known isoforms of Akt in mammalian cells [Akt1 (alpha), Akt2 (beta) and Akt3 (γ)]; Akt is activated by insulin and growth and survival factors. Akt1 is phosphorylated at Thr308 and Ser473.
Additionally, Akt has been referred to as an oncogene because it has increased activity in a number of tumors. - See more at: http://www.avivasysbio.com/sd/tds/html_datasheet.php?sku=OAAI00738
Phospho-peptide, non-phospho-peptide
Dot Blot: 1 ?g peptide was blot onto NC membrane, Lane A and C: Phospho-Peptides 1- AKT1 (pThr308); 2- AKT1(pSer473); 3- Non-related phosphopeptides 4- Non-related phosphopeptides Lane B and D: Non-Phospho-Peptides 1- AKT1 (Non phosphorylated at 308); 2- AKT1(Non-phosphorylated at 473); 3- Non-related Non-phosphopeptides 4- Non-related Non-phosphopeptides Column 2: Non-Phospho Peptide AKT1 (T308) Column 3 : Peptide AKT1 (pThr308) Lane A and B were blotted by Rabbit anti-AKT1(pT308) (Cat#OAAI00382) at a 1:2000 dilution. Lane C and D were blotted by Rabbit anti-AKT1(Paired T308) (Cat#OAAI00738) at a 1:2000 dilution.