The antibodies are stable for 12 months from date of receipt when stored at -20C to -70C. The antibodies can be stored at 2C to 8C for three month without detectable loss of activity. Avoid repeated freezing thawing cycles.
亚型:
Rabbit IgG
免疫原:
A synthetic peptide corresponding to the epitope SGIHS without phosphorylation of Human beta-catenin.
规格:
100 ug
NCBI Gene Id: 1499
Swissprot Id: P35222
Beta-catenin is a cytosolic, 88 kDa, 781 amino acid protein belongs to the beta-catenin family. The N-terminus domain, containing the binding site and the phosphorylation sites. Beta-Catenin serves as a link between cytoskeleton actin and transmembrane cadherin(s). It is believed to contribute to tight cell-to-cell adhesion. It can enter the nucleus and interact with the TCF/LEF family of transcription factors, initiating gene expression. Normally, beta-catenin transcriptional activity is suppressed by a Ser/Thr kinase termed GSK3beta and/or Casein Kinase I (CK1). Kinases are constitutively active and phosphorylates beta-catenin at multiple sites, including S33 and S37, Y96, Y228, Y280 etc. Phosphorylation of beta-catenin targets the molecule for degradation via a ubiquination-mediated pathway. GSK3beta activity can be blocked by upstream signaling events such as Wnt-Frizzled interaction. This inhibits GSK3beta, allowing unphosphorylated beta-catenin to enter the nucleus and initiate gene activation. The phosphorylation of beta-catenin might contribute to tumorigenesis. - See more at: http://www.avivasysbio.com/sd/tds/html_datasheet.php?sku=OAAI00596
Western Blot: The cell lysate derived from HELA was immunoprobed at a dilution of 1:500 by the following antibodies: 1: Rabbit anti-beta-Catenin (pS33/S37) (Cat#OAAI00538) 2: Rabbit anti-beta-Catenin (pS33) (Cat#OAAI00537) 3: Rabbit anti-beta-Catenin (pS37) (Cat#OAAI00597) 4: Rabbit anti-beta-Catenin (Non-phospho) (Cat#OAAI00596) 5: Negative control.