The antibodies are stable for 12 months from date of receipt when stored at -20C to -70C. The antibodies can be stored at 2C to 8C for three month without detectable loss of activity. Avoid repeated freezing thawing cycles.
亚型:
Rabbit IgG
免疫原:
A synthetic peptide corresponding to the C-term of epitope KKKSG at a phosphorylation Ser188 of Human Rho protein
规格:
100 ug
NCBI Gene Id: 387
Swissprot Id: P61586
Ras homolog gene family consists of RhoA, RhoB, RhoC proteins. These proteins interact directly with protein kinase (Rho kinase) to serve as downstream effectors of the activated GTPase. The Rho proteins are involved in the regulation of the actin cytosekeleton. Activation of Rho to the GTP bound state promotes formation of stress fibers and is required for the formation and maintenance of focal adhesions. Pathways stimulated by cytokines, hormones and various forms of stress utilize p21 proteins of the Rho family2. Possible mediators for these responses include protein kinase such as p120PKN, and p150, ROKalpha/Rho-A kinase, myosin light chain (MLC), phosphatase and PIP 5-kinase.3 - See more at: http://www.avivasysbio.com/sd/tds/html_datasheet.php?sku=OAAI00595
Phospho-peptide, non-phospho-peptide
Dot Blot: 1 ?g peptide was blot onto NC membrane (A- Rho(pS188); B-Rho (Nonphospho); C: Non- related Phosphopeptide) was blotted by Rabbit anti- Rho (pS188) (Cat#OAAI00595) at a 1:2000 dilution.
conditioned MCF-7
WB: The cell lysate derived from conditioned MCF-7 was immunoprobed by Rabbit anti-Rho (pS188) (Cat#OAAI00595) at 1:500. An immunoreactive band is observed around ~24 kDa (1). This band is abolished by pre-incubation with immunizing peptide (2).