The antibodies are stable for 12 months from date of receipt when stored at -20C to -70C. The antibodies can be stored at 2C to 8C for three month without detectable loss of activity. Avoid repeated freezing thawing cycles.
亚型:
Rabbit IgG
免疫原:
A synthetic peptide corresponding to the epitope - PLSQE- at a phosphorylation site at serine 15 of Human p53 protein
规格:
100 ug
NCBI Gene Id: 7157
Swissprot Id: P04637
P53, also known as protein 53, or tumor suppressor protein 53, plays important roles in cellular response to DNA damage and other genomic aberRation. Mutations in the p53 tumor suppressor gene giving rise to mutant p53 proteins are among the most common genetic alteRations associated with tumor cells. P53 protein is phosphrylated at multiple sites in vivo and in vitro in response to different stimuli. UV caused DNA damage can induce Ser9, Ser15, Ser20 phosphorylation while ATM, ATR, DNA-PK at Ser15 and Ser37. - See more at: http://www.avivasysbio.com/sd/tds/html_datasheet.php?sku=OAAI00414
UV treated Hela
IHC: UV treated Hela cells cultured in the cell chamber, fixed, and immunoprobed by Rabbit Anti-p53 (pS15) antibody, (Cat#OAAI00414) at 1:100 for 10 min @ RT.
UV stimulated Hela
WB: The cell lysate derived from UV stimulated Hela cells was immunoprobed by Rabbit anti-p53 (pS15) (cat#OAAI00414) at 1:500. An immunoreactive band is observed around ~53kDa (Lane 1). The lane 2 is a negative control.