The antibodies are stable for 12 months from date of receipt when stored at -20C to -70C. The antibodies can be stored at 2C to 8C for three month without detectable loss of activity. Avoid repeated freezing thawing cycles.
亚型:
Rabbit IgG
免疫原:
A synthetic peptide surrounding to the epitope -PMSPR- with phosphorylation sites at Ser727 of STAT3 protein from Human, Mouse, Rat, chicken dog and bovine origins.
规格:
100 ug
NCBI Gene Id: 6774
Swissprot Id: P40763
STAT proteins (Signal transducer and activator of transcription) belong to a family of cytoplasmic transcription factors that can be phosphorylated by a lignad binding to its cell surface receptor. The phosphorylation of STAT3 induces STAT dimerization, nuclear translocation and DNA binding. Stat3 is constitutively activated in a number of Human tumors. Stat3- activated transcription seems to be regulated by serine phosphorylation at Ser727.
- See more at: http://www.avivasysbio.com/sd/tds/html_datasheet.php?sku=OAAI00081
Phospho-peptide, non-phospho-peptide
Dot Blot: 1 ?g peptide was blot onto NC membrane A: STAT3 (pS727) (Phosphospecific) B: STAT3 (Paired S727) (Non phosphospeicfic) were blotted at a 1:2000 dilution by: 1: Rabbit anti-STAT3 (Paired S727) (Cat#OAAI00211), 2: Rabbit anti-STAT3 (Phospho specific), (Cat#OAAI00081).
A431
Western Blot: The whole cell lysate derived from A431 with EGF stimulated (Lane 1) or without EGF stimulated (Lane 2) were immunoblotted by Rabbit anti STAT3 (pS727) (Cat#OAAI00081) antibody at 1:500.