All lanes : Peptidyl-prolyl cis-trans isomerase FKBP1A antibody at at 2 µg/ml Lane 1 :EC109 whole cell lysate Lane 2 :293T whole cell lysate Secondary Goat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size : 12 kDa Observed band size: 22 kDa Additional bands at : 36 kDa. We are unsure as to the identity of these extra band.