All lanes : Prefoldin subunit 5 antibody at at 2 µg/ml Lane 1 :EC109whole cell lysate Lane 2 :293T whole cell lysate Secondary Goat polyclonal to Rabbit IgG at 1/15000 dilutionPredicted band size : 17 kDaObserved band size : 70 kDa Additional bands at : 80kDa. We are unsure as to the identity of these extra band.