All lanes : Thiopurine S-methyltransferase antibody at at 2 µg/ml Lane 1 :EC109 whole cell lysate Lane 2 :293T whole cell lysate Secondary Goat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size : 27kDa Observed band size: 32kDa Additional bands at : 90 kDa, 100 kDa; We are unsure as to the identity of these extra bands.