The antiserum was produced against synthesized peptide derived between 30-79 amino acids from the Internal region of human 4E-BP1 around the phosphorylation site of Ser64.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using 4E-BP1 (Phospho-Ser64) Antibody. The picture on the right is treated with the synthesized peptide.
Jurkat cells
Western blot analysis of extracts from Jurkat cells treated with Insulin 0.01U/ml 15', using 4E-BP1 (Phospho-Ser64) Antibody. The lane on the right is treated with the synthesized peptide.