The antiserum was produced against synthesized peptide derived between 8-57 amino acids from the N-Terminal region of human LEF-1 around the phosphorylation site of Ser42.
Western blot analysis of extracts from 293 cells treated with paclitaxel 1uM 24h, using LEF-1 (Phospho-Ser42) Antibody. The lane on the right is treated with the synthesized peptide.