The antiserum was produced against synthesized non-phosphopeptide derived from human 4E-BP1 around the phosphorylation site of threonine 45 (S-T-TP-P-G).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 4E-BP1 (Ab-45) antibody (OAEC00557).
MDA-MB-435
Western blot analysis of extracts from MDA-MB-435 cells untreated or treated with EGF (200ng/ml, 5min), using 4E-BP1 (Ab-45) antibody (OAEC00557, Lane1 and 2) and 4E-BP1 (phospho-Thr45) antibody (OAEC00208, Lane 3 and 4).